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human cd45 positive 152 selection kit  (Miltenyi Biotec)


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    Miltenyi Biotec human cd45 positive 152 selection kit
    Human Cd45 Positive 152 Selection Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+positive+selection+kit/pm41672197-73-37-43?v=Miltenyi+Biotec
    Average 96 stars, based on 398 article reviews
    human cd45 positive 152 selection kit - by Bioz Stars, 2026-07
    96/100 stars

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    (A) Experimental outline for gene expression analysis of immune cells <t>(CD45+)</t> from draining lymph nodes. C57BL/6 mice were immunized intramuscularly with each respective vaccine, and at 24 h post-immunization, draining lymph nodes were enriched for CD45+ cells using magnetic beads. ( B ) Venn diagram showing the overlap of significant differentially expressed genes (DEGs) among each vaccine group compared to naive controls. Significance was defined as FDR < 0.05 and absolute log2 fold change > 0.5. ( C ) Bar plot depicting the proportions of annotated cell types in each vaccination group. ( D ) UMAP visualization of single-cell transcriptomes, with cells colored and labeled by cell identity. UMAPs are shown separately for each vaccination group. ( E-G ). Volcano plots displaying DEGs for Ad5 vs. naive, mRNA vs. naive, and protein vs. naive comparisons. Vertical lines indicate log2 fold change of 0.5; horizontal lines indicate FDR of 0.05. Genes upregulated in the vaccine group are shown in red, downregulated in blue, and non-significant genes in grey. ( H ) UMAP plots showing the expression of Ifit3 across each vaccination group and naive mice. ( I–L ) Dot plots of enriched pathways identified by GSEA in B cells ( I ), CD4 T cells ( J ), CD8 T cells ( K ), and dendritic cells ( L ) for each vaccine group compared to naive. The y-axis lists pathway names, dot size represents –log10(adjusted p-value), and color corresponds to the normalized enrichment score (NES). A black ring denotes significant enrichment (FDR < 0.05); a grey ring indicates non-significance.
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    (A) Experimental outline for gene expression analysis of immune cells <t>(CD45+)</t> from draining lymph nodes. C57BL/6 mice were immunized intramuscularly with each respective vaccine, and at 24 h post-immunization, draining lymph nodes were enriched for CD45+ cells using magnetic beads. ( B ) Venn diagram showing the overlap of significant differentially expressed genes (DEGs) among each vaccine group compared to naive controls. Significance was defined as FDR < 0.05 and absolute log2 fold change > 0.5. ( C ) Bar plot depicting the proportions of annotated cell types in each vaccination group. ( D ) UMAP visualization of single-cell transcriptomes, with cells colored and labeled by cell identity. UMAPs are shown separately for each vaccination group. ( E-G ). Volcano plots displaying DEGs for Ad5 vs. naive, mRNA vs. naive, and protein vs. naive comparisons. Vertical lines indicate log2 fold change of 0.5; horizontal lines indicate FDR of 0.05. Genes upregulated in the vaccine group are shown in red, downregulated in blue, and non-significant genes in grey. ( H ) UMAP plots showing the expression of Ifit3 across each vaccination group and naive mice. ( I–L ) Dot plots of enriched pathways identified by GSEA in B cells ( I ), CD4 T cells ( J ), CD8 T cells ( K ), and dendritic cells ( L ) for each vaccine group compared to naive. The y-axis lists pathway names, dot size represents –log10(adjusted p-value), and color corresponds to the normalized enrichment score (NES). A black ring denotes significant enrichment (FDR < 0.05); a grey ring indicates non-significance.
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    (A) Experimental outline for gene expression analysis of immune cells (CD45+) from draining lymph nodes. C57BL/6 mice were immunized intramuscularly with each respective vaccine, and at 24 h post-immunization, draining lymph nodes were enriched for CD45+ cells using magnetic beads. ( B ) Venn diagram showing the overlap of significant differentially expressed genes (DEGs) among each vaccine group compared to naive controls. Significance was defined as FDR < 0.05 and absolute log2 fold change > 0.5. ( C ) Bar plot depicting the proportions of annotated cell types in each vaccination group. ( D ) UMAP visualization of single-cell transcriptomes, with cells colored and labeled by cell identity. UMAPs are shown separately for each vaccination group. ( E-G ). Volcano plots displaying DEGs for Ad5 vs. naive, mRNA vs. naive, and protein vs. naive comparisons. Vertical lines indicate log2 fold change of 0.5; horizontal lines indicate FDR of 0.05. Genes upregulated in the vaccine group are shown in red, downregulated in blue, and non-significant genes in grey. ( H ) UMAP plots showing the expression of Ifit3 across each vaccination group and naive mice. ( I–L ) Dot plots of enriched pathways identified by GSEA in B cells ( I ), CD4 T cells ( J ), CD8 T cells ( K ), and dendritic cells ( L ) for each vaccine group compared to naive. The y-axis lists pathway names, dot size represents –log10(adjusted p-value), and color corresponds to the normalized enrichment score (NES). A black ring denotes significant enrichment (FDR < 0.05); a grey ring indicates non-significance.

    Journal: bioRxiv

    Article Title: Dosing and Serostatus Shape the Efficacy of Adenovirus, mRNA, and Protein Vaccines

    doi: 10.1101/2025.07.16.665159

    Figure Lengend Snippet: (A) Experimental outline for gene expression analysis of immune cells (CD45+) from draining lymph nodes. C57BL/6 mice were immunized intramuscularly with each respective vaccine, and at 24 h post-immunization, draining lymph nodes were enriched for CD45+ cells using magnetic beads. ( B ) Venn diagram showing the overlap of significant differentially expressed genes (DEGs) among each vaccine group compared to naive controls. Significance was defined as FDR < 0.05 and absolute log2 fold change > 0.5. ( C ) Bar plot depicting the proportions of annotated cell types in each vaccination group. ( D ) UMAP visualization of single-cell transcriptomes, with cells colored and labeled by cell identity. UMAPs are shown separately for each vaccination group. ( E-G ). Volcano plots displaying DEGs for Ad5 vs. naive, mRNA vs. naive, and protein vs. naive comparisons. Vertical lines indicate log2 fold change of 0.5; horizontal lines indicate FDR of 0.05. Genes upregulated in the vaccine group are shown in red, downregulated in blue, and non-significant genes in grey. ( H ) UMAP plots showing the expression of Ifit3 across each vaccination group and naive mice. ( I–L ) Dot plots of enriched pathways identified by GSEA in B cells ( I ), CD4 T cells ( J ), CD8 T cells ( K ), and dendritic cells ( L ) for each vaccine group compared to naive. The y-axis lists pathway names, dot size represents –log10(adjusted p-value), and color corresponds to the normalized enrichment score (NES). A black ring denotes significant enrichment (FDR < 0.05); a grey ring indicates non-significance.

    Article Snippet: At day 1 post-vaccination, lymph nodes from five mice were pooled and MACS-sorted with a CD45 MACS positive selection kit (STEMCELL) and used for single-cell sequencing.

    Techniques: Gene Expression, Magnetic Beads, Labeling, Expressing